Search results for " PFGE"

showing 6 items of 6 documents

Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

2018

Abstract Staphylococcus aureus is the major cause of foodborne diseases worldwide. In this retrospective study, 84 S. aureus strains were characterized. The collection comprises 78 strains isolated during 1998 and 2014 from dairy products and tissue samples from livestock bred for dairy production in Sicily. One isolate was obtained from a pet (dog), one from an exotic animal (a circus elephant), and four human isolates were obtained during a severe food poisoning outbreak that occurred in Sicily in 2015. All the strains were characterized by pulsed-field gel electrophoresis (PFGE), for antibiotic resistance and presence of toxin genes. PFGE results showed 10 different pulsotypes, with thre…

0301 basic medicineStaphylococcus aureusLivestockantibiotic resistanceGenotypeMLST; MRSA; PFGE; Staphylococcus aureus; antibiotic resistance; toxin genesTetracycline030106 microbiologyVirulenceMRSABiologymedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyMicrobiologyMicrobiologyFoodborne DiseasesEnterotoxins03 medical and health sciencesAntibiotic resistanceDrug Resistance BacterialPulsed-field gel electrophoresismedicineAnimalsHumansSicilyRetrospective StudiesVirulenceOutbreakOriginal ArticlesPFGEStaphylococcal InfectionsAnti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldPenicillin030104 developmental biologyStaphylococcus aureustoxin genesStaphylococcus aureuFood MicrobiologyMultilocus sequence typingAnimal Science and ZoologyMultilocus Sequence TypingMLSTFood Sciencemedicine.drug
researchProduct

Uso combinato di SBT e PFGE quali metodi molecolari per la tipizzazione di stipiti di Legionella pneumophila sierogruppo 1

2008

Legionella SBT PFGE
researchProduct

Salmonella bongori48:z35:– in Migratory Birds, Italy

2009

Suggested citation for this article: Foti M, Daidone A, Aleo A, Pizzimenti A, Giacopello C, Mammina C. Salmonella bongori 48:z35:– in migratory birds, Italy. Emerg Infect Dis [serial on the Internet]. 2009 Mar [date cited]. Available from http://www.cdc.gov/EID/content/15/3/502.htm

Microbiology (medical)Salmonella bongoriSerotypeSalmonellaEpidemiologyletterlcsh:MedicineSettore MED/42 - Igiene Generale E Applicatamedicine.disease_causeSalmonella bongorilcsh:Infectious and parasitic diseasesBirdsSalmonellaPrevalencemedicineAnimalslcsh:RC109-216Salmonella bongori; wild birds; PFGE; epidemiologySerotypingLetters to the Editorwild birdsBird DiseasesSalmonella Infections AnimalbiologyBird Diseaseslcsh:RPFGESalmonella bongori migratory birds epidemiologybiology.organism_classificationVirologyElectrophoresis Gel Pulsed-FieldInfectious DiseasesItalyAnimal MigrationEmerging Infectious Diseases
researchProduct

A combined molecular typing approach does not discriminate Legionella pneumophila serogroup 1 strains of a predominant sequence-based type in Palermo…

2009

Summary: The sequence-based type 1,4,3,1,1,1 of Legionella pneumophila sg.1 is predominant in the Palermo city environment since several years. In this study, extended sequence-based typing and pulsed field gel electrophoresis were used in a combined approach in the aim to enhance discriminatory power of the molecular typing procedures. However, probably due to a common environmental reservoir and genetic stability, most of the strains circulating in the geographic area under study belong to the same clone and are, consequently, indistinguishable by molecular typing. Investigations of clinical cases and tracing to their environmental source require caution and support from sound epidemiolog…

Settore MED/07 - Microbiologia E Microbiologia ClinicaGenotypeEpidemiologyLegionella pneumophila Molecular typing Epidemiology SBT PFGEMolecular typingLegionella pneumophilaLegionella pneumophilaMicrobiologylcsh:Infectious and parasitic diseasesMolecular typingType (biology)Pulsed-field gel electrophoresisHumansSBTlcsh:RC109-216TypingLegionella pneumophila Serogroup 1Sequence (medicine)biologylcsh:Public aspects of medicineSputumPublic Health Environmental and Occupational Healthlcsh:RA1-1270PFGEGeneral Medicinebiology.organism_classificationCombined approachElectrophoresis Gel Pulsed-FieldInfectious DiseasesItalyLegionnaires' DiseaseWater MicrobiologySequence AnalysisJournal of Infection and Public Health
researchProduct

Use of pulsed field gel electrophoresis (PFGE)and single-enzyme amplified fragment length polymorphism (SE-AFLP) to subtype isolates of Salmonella en…

2005

Settore MED/07 - Microbiologia E Microbiologia ClinicaSalmonella enterica PFGESettore MED/42 - Igiene Generale E Applicata
researchProduct

Use of pulsed field gel electrophoresis (PFGE) and single-enzyme amplified fragment lenght polymorphism (SE-AFLP) to subtype isolates of Salmonella e…

2005

Settore MED/07 - Microbiologia E Microbiologia ClinicaSettore MED/42 - Igiene Generale E ApplicataSalmonella enterica PFGE AFLP
researchProduct